Ambion megaclear manual

Manual M Revision B Revision Date: January 4, For research use only. Not for use in diagnostic procedures. Literature Citation When describing a procedure for publication using this product, we would appreciate D. Related Products Available from Ambion MEGAclear™. MEGAclear™ Kit User Guide 5 Quality control Functional testing Quality control Functional testingA µL MEGAscript® reaction is split into 10 samples and purified in 10 random wells on a single MEGAclear™ Filter www.doorway.rury is shown to be 75% .  · MEGAclear™ P/N AM MEGAclear purifies RNA from transcription, and other enzymatic reactions yielding high quality RNA free of unincorporated nucleotides, enzymes and buffer components with close to % recovery of input RNA. RNaseZap® Solution P/N AM, AM, AM RNaseZap RNase Decontamination Solution is simply sprayed, poured, or.

Ambion RNase-free tubes and tips are available in most commonly used sizes and styles. They are guaranteed RNase- and DNase-free. See our latest catalog for specific information. TURBO DNA-free™ P/N AM The TURBO DNA-free Kit employs Ambion’s exclusive TURBO DNase (patent pending); a specifically engineer ed hyperactive DNase that. E. Related Products Available from Ambion MEGAclear™ Cat # MEGAclear purifies RNA from MEGAscript, and other enzymatic reactions yielding high quality RNA free of unincorporated nucleotides, enzymes and buffer components with close to % recovery of input RNA. MEGAclear™ Automated Cat # The MEGAclear™ Kit procedure consists of three steps: 1. RNA is bound to the glass fiber in the Filter Plate. 2. Contaminants are washed away. 3. RNA is eluted in a low salt buffer or in Nuclease-free Water. The MEGAclear™ Kit can be used to remove nucleotides, short oligonucleotides, proteins, and salts from RNA.

Purify the Cas9 mRNA using the MEGAclear Transcription Clean-Up Kit according to the manufacturer's instructions, and elute it with 65 µL of elution buffer. (Ambion) according to the manufacturer's manual. MEGAclear Transcription Clean-Up Kit (Ambion). mercaptoethanol (Invitrogen) for 14 days. the RNAprotect manual. ada), and MEGAclear kit (Applied Biosystems/Ambion), were performed according to the manufacturer's instructions.